CD19 Protein (AA 21-291) (His tag)
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Quick Overview for CD19 Protein (AA 21-291) (His tag) (ABIN7274055)
抗原
See all CD19 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 21-291
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标记
- This CD19 protein is labelled with His tag.
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原理
- Cynomolgus/Rhesus macaque CD19 Protein
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序列
- Gln21-Trp291
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产品特性
- Recombinant Cynomolgus/Rhesus macaque CD19 Protein is expressed from HEK293 with His tag at the N-terminus, It contains Gln21-Trp291.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.46 uM as determined in SPR assay (Biacore T200).,Cynomolgus/Rhesus macaque CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-CD19 Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.27 μM as determined in BLI assay (Gator® Prime).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- CD19 (CD19 Molecule (CD19))
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别名
- CD19
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背景
- CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores.
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分子量
- 30.96 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
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UniProt
- A0A2K5W8L9
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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