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CD19 Protein (AA 21-291) (His tag)

This Recombinant CD19 protein is expressed in HEK-293 Cells.
产品编号 ABIN7274055
发货至: 中国
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Quick Overview for CD19 Protein (AA 21-291) (His tag) (ABIN7274055)

抗原

See all CD19 蛋白
CD19 (CD19 Molecule (CD19))

蛋白类型

Recombinant

生物活性

Active

宿主

  • 32
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猕猴, Cynomolgus

资源

  • 31
  • 5
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HEK-293 Cells

纯度

> 95 % as determined by Bis-Tris PAGE
  • 产品特性

    AA 21-291

    标记

    This CD19 protein is labelled with His tag.

    原理

    Cynomolgus/Rhesus macaque CD19 Protein

    序列

    Gln21-Trp291

    产品特性

    Recombinant Cynomolgus/Rhesus macaque CD19 Protein is expressed from HEK293 with His tag at the N-terminus, It contains Gln21-Trp291.

    过滤

    0.22 μm filtered

    内毒素水平

    Less than 1 EU per μg by the LAL method.

    Biological Activity Comment

    Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.46 uM as determined in SPR assay (Biacore T200).,Cynomolgus/Rhesus macaque CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-CD19 Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.27 μM as determined in BLI assay (Gator® Prime).
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

    缓冲液

    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    储存条件

    -20 °C,-80 °C

    储存方法

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    CD19 (CD19 Molecule (CD19))

    别名

    CD19

    背景

    CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores.

    分子量

    30.96 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

    UniProt

    A0A2K5W8L9

    途径

    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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