EpCAM Protein (AA 24-265) (His-Avi Tag,Biotin)
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- 抗原 See all EpCAM (EPCAM) 蛋白
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-265
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This EpCAM protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human EpCAM/TROP1 Protein
- 序列
- Gln24-Lys265
- 产品特性
- Recombinant Biotinylated Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Lys265.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human EpCAM, His Tag at 0.2μg/ml (100ul/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA. See testing image for detail.
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Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (His tag)
EPCAM 宿主: 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (His tag)EPCAM 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (Fc Tag)EPCAM 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (His-DYKDDDDK-Strep II Tag)EPCAM 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Epithelial Cell Adhesion Molecule (EPCAM) (Active) protein (Fc Tag)EPCAM 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Epithelial Cell Adhesion Molecule (EPCAM) (AA 118-314) protein (His tag)EPCAM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Epithelial Cell Adhesion Molecule (EPCAM) (AA 1-314) protein (GST tag)EPCAM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Epithelial Cell Adhesion Molecule (EPCAM) (Active) protein (His tag)EPCAM 宿主: 人 宿主: HEK-293 Cells Recombinant > 96 % as determined by reducing SDS-PAGE. Active
Epithelial Cell Adhesion Molecule (EPCAM) proteinEPCAM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % pure SDS
Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) protein (His tag,AVI tag,Biotin)EPCAM 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 别名
- EpCAM (EPCAM 产品)
- 背景
- Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF-like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
- 分子量
- 30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
- UniProt
- P16422
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