BST2 Protein (AA 49-161) (His tag)
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- 抗原 See all BST2 蛋白
- BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 49-161
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This BST2 protein is labelled with His tag.
- 原理
- Human BST2 Protein
- 序列
- Asn49-Ser161
- 产品特性
- Recombinant Human BST2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn49-Ser161.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human BST2, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 15.9ng/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))
- 别名
- BST2 (BST2 产品)
- 别名
- BST2 Protein, CD317 Protein, TETHERIN Protein, 2310015I10Rik Protein, Bst-2 Protein, C87040 Protein, DAMP-1 Protein, GREG Protein, Damp1 Protein, bone marrow stromal cell antigen 2 Protein, bone marrow stromal antigen 2 Protein, BST2 Protein, LOC618737 Protein, Bst2 Protein
- 背景
- Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.
- 分子量
- 14.5 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
- 途径
- Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response
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