BST2 Protein (AA 49-161) (His tag)
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Quick Overview for BST2 Protein (AA 49-161) (His tag) (ABIN7273992)
抗原
See all BST2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 49-161
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标记
- This BST2 protein is labelled with His tag.
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原理
- Human BST2 Protein
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序列
- Asn49-Ser161
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产品特性
- Recombinant Human BST2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn49-Ser161.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human BST2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).,Human BST2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))
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别名
- BST2
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背景
- Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.
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分子量
- 14.5 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
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UniProt
- Q10589
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途径
- Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response
抗原
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