CD86 Protein (CD86) (AA 26-247) (His-Avi Tag)
Quick Overview for CD86 Protein (CD86) (AA 26-247) (His-Avi Tag) (ABIN7273898)
抗原
See all CD86 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 26-247
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标记
- This CD86 protein is labelled with His-Avi Tag.
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原理
- Human B7-2/CD86 Protein
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序列
- Leu26-Pro247
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产品特性
- Recombinant Human B7-2/CD86 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Pro247.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
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CD86 (CD86) (AA 26-247) protein (Fc Tag)
CD86 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
CD86 (CD86) (AA 26-240) protein (His tag)CD86 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD86 (CD86) (AA 19-240) protein (His tag)HPLC verified CD86 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
CD86 (CD86) (AA 26-247) (Active) protein (Fc Tag)CD86 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD86 (CD86) (AA 26-247) protein (His-Avi Tag,Biotin)CD86 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD86 (CD86) (AA 24-246) protein (His-DYKDDDDK-Strep II Tag)CD86 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD86 (CD86) (AA 24-245) (Active) protein (Fc Tag)CD86 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD86 (CD86) (AA 26-247) (Active) protein (Fc Tag,AVI tag,Biotin)CD86 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD86 (CD86) protein (His tag)CD86 宿主: 猕猴 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
CD86 (CD86) protein (His tag)CD86 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- CD86
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别名
- B7-2
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背景
- B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
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分子量
- 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
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UniProt
- P42081-1
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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