TNFRSF10B Protein (His tag,Fc Tag)
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Quick Overview for TNFRSF10B Protein (His tag,Fc Tag) (ABIN7198417)
抗原
See all TNFRSF10B 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This TNFRSF10B protein is labelled with His tag,Fc Tag.
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原理
- Recombinant Mouse TRAIL R2/TNFRSF10B Protein (His & Fc Tag)(Active)
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序列
- Met 1-Ser 177
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产品特性
- A DNA sequence encoding the extracellular domain of mouse TNFRSF10B (NP_064671.2) (Met 1-Ser 177) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- 1. Immobilized human TNFSF10 at 10 μg/ml (100 μl/well) can bind mouse TNFRSF10B-Fch, The EC50 of mouse TNFRSF10B-Fch is 0.07-0.17 μg/ml.2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 20-80 ng/ml in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.
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Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 56-182) (Active) protein (Biotin,His-Avi Tag)
TNFRSF10B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 56-182) (Active) protein (hFc Tag)TNFRSF10B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 56-182) (Active) protein (His-Avi Tag)TNFRSF10B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 53-180) (Active) protein (His tag)TNFRSF10B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 56-180) protein (His-DYKDDDDK-Strep II Tag)TNFRSF10B 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 56-210) protein (His-DYKDDDDK-Strep II Tag)TNFRSF10B 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 56-182) protein (mFc Tag)TNFRSF10B 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (AA 56-182) (Active) protein (Fc Tag)TNFRSF10B 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) protein (His tag,AVI tag)TNFRSF10B 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) (Active) protein (His tag)TNFRSF10B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
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别名
- TRAIL R2/TNFRSF10B
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背景
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Background: Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: Tumor Necrosis Factor Receptor Superfamily Member 10B; Death Receptor 5; TNF-Related Apoptosis-Inducing Ligand Receptor 2; TRAIL Receptor 2; TRAIL-R2; CD262; TNFRSF10B; DR5; KILLER; TRAILR2; TRICK2; ZTNFR9
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分子量
- 41.8 kDa
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NCBI登录号
- NP_064671
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途径
- p53 Pathway, Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
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