TEK Protein (AA 770-1122) (His-GST)
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Quick Overview for TEK Protein (AA 770-1122) (His-GST) (ABIN7320054)
抗原
See all TEK 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 770-1122
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标记
- This TEK protein is labelled with His-GST.
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原理
- Recombinant Mouse Tie2/CD202b Protein (aa 770-1122, His & GST Tag)
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序列
- Gln770-Ala1122
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产品特性
- A DNA sequence encoding the mouse TEK (Q02858) (Gln770-Ala1122) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- 1. No Kinase Activity2. Immobilized mouse TEK (aa 770-1122) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 0.25-2.0 μg/ml.
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TEK Tyrosine Kinase, Endothelial (TEK) (AA 23-748) (Active) protein (Fc Tag)
TEK 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
TEK Tyrosine Kinase, Endothelial (TEK) (AA 23-745) protein (His tag)TEK 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
TEK Tyrosine Kinase, Endothelial (TEK) (AA 1-744) (Active) protein (His tag)TEK 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. ELISA Active
TEK Tyrosine Kinase, Endothelial (TEK) (Active) protein (Fc Tag)TEK 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. ELISA Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
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别名
- Tie2/CD202b
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背景
- AA517024,Cd202b,Hyk,STK1,Tie-2,Tie2,TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
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分子量
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Calculated MW: 68.2 kDa
Observed MW: 68 kDa
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基因ID
- 21687
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UniProt
- Q02858
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途径
- RTK signaling, Growth Factor Binding
抗原
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