SERPIND1 Protein (His tag)
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Quick Overview for SERPIND1 Protein (His tag) (ABIN7197974)
抗原
See all SERPIND1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This SERPIND1 protein is labelled with His tag.
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原理
- Recombinant Mouse SerpinD1/HCF2 Protein (His Tag)(Active)
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序列
- Met1-Ser 478
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产品特性
- A DNA sequence encoding the mouse SerpinD1 precursor (NP_032249.3) (Met1-Ser 478) was expressed, with a C-terminal polyhistidine tag.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to inhibit thombin (Sigma, T4648) cleavage of fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catslog# ES011). The IC50 value is < 1.2 nM.
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serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) protein (His tag)
SERPIND1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) (AA 1-499) protein (GST tag)SERPIND1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) (AA 58-499) protein (His tag)SERPIND1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 25 mM HEPES, 150 mM NaCl, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))
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别名
- SerpinD1/HCF2
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背景
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Background: SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Synonym: Heparin cofactor 2; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; Serpin D1
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分子量
- 53.5 kDa
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NCBI登录号
- NP_032249
抗原
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