SERPIND1 Protein (His tag)
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Quick Overview for SERPIND1 Protein (His tag) (ABIN7197973)
抗原
See all SERPIND1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This SERPIND1 protein is labelled with His tag.
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原理
- Recombinant Human SerpinD1 Protein (His Tag)(Active)
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序列
- Met 1-Ser 499
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产品特性
- A DNA sequence encoding the human SerpinD1 precursor (NP_000176.2) (Met 1-Ser 499) was expressed with a C-terminal polyhistidine tag.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized recombinant human SerpinD1-His at 10 μg/ml (100 μl/well) can bind biotinylated recombinant mouse ELANE-His with a linear range of 0.15-10.0 μg/ml.
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serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) protein (His tag)
SERPIND1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) (AA 1-499) protein (GST tag)SERPIND1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) (AA 58-499) protein (His tag)SERPIND1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) (Active) protein (His tag)SERPIND1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))
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别名
- SerpinD1
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背景
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Background: SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Synonym: D22S673;HC2;HCF2;HCII;HLS2;LS2;SerpinD1;THPH10
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分子量
- 56.4 kDa
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NCBI登录号
- NP_000176
抗原
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