Renin Protein (REN) (His tag)
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Quick Overview for Renin Protein (REN) (His tag) (ABIN7320334)
抗原
See all Renin (REN) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Renin protein is labelled with His tag.
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原理
- Recombinant Mouse REN1/Renin-1 Protein (His Tag)(Active)
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序列
- Met 1-Arg 402
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产品特性
- A DNA sequence encoding the pro form of mouse REN1 (NP_112469.1) (Met 1-Arg 402) was fused with a polyhistidine tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- 1. Measured by its ability to cleave the fluorogenic peptide substrate 5-FAM/QXL™ 520 (Peti-Peterdi, J. et al., 2009, Physiology 24:88.). The specific activity is > 20 pmoles/min/μg.2. Immobilized mouse REN1-His at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His with a linear range of 31.25-250 ng/ml.
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Renin (REN) protein (His tag)
REN 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Renin (REN) (AA 24-406) protein (GST tag)REN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Renin (REN) (AA 24-406) protein (His tag)REN 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Renin (REN)
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别名
- REN1/Renin-1
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背景
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Background: Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.
Synonym: Renin-1; Angiotensinogenase; Kidney renin; Ren1; Ren; Ren-1;Angiotensin-forming enzyme;Ren-A;Ren1c;Ren1d;Rn-1;Rnr
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分子量
- 43.2 kDa
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NCBI登录号
- NP_112469
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
抗原
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