PLAT 蛋白
Quick Overview for PLAT 蛋白 (ABIN7197374)
抗原
See all PLAT 蛋白蛋白类型
宿主
资源
纯度
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原理
- Recombinant Human tPA/PLAT Protein
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序列
- Ile 311-Pro 562
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产品特性
- The β chain (Ile 311-Pro 562) of mature human tPA (NP_000921.1) was obtained after cleavage of the N-terminal human IgG1 Fc region from the purified chimera.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Plasminogen Activator, Tissue (PLAT) (AA 1-562) protein (GST tag)
PLAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Plasminogen Activator, Tissue (PLAT) (AA 24-562) protein (His tag)PLAT 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
Plasminogen Activator, Tissue (PLAT) (AA 36-310) protein (His tag)PLAT 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- PLAT (Plasminogen Activator, Tissue (PLAT))
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别名
- tPA/PLAT
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背景
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Background: Tissue plasminogen activator (abbreviated tPA or PLAT), is traditionally viewed as a simple serine protease whose main function is to convert plasminogen into biologically active plasmin. As a protease, tPA plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. tPA is synthesized and secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Proteolytic cleavage at the C-terminal side of Arg275 generates the enzyme composed of two subunits, designated as α and β chains which are held together by a single disulfide bond. Unlike the other members of the chymotrypsin family, tPA has one particular distinction in that the catalytic efficiency of the single-chain enzyme is only slightly lower than that of the proteolytically cleaved form and is therefore not a true zymogen. tPA is found not only in the blood, where its primary function is as a thrombolytic enzyme, but also in the central nervous system (CNS). It participats in a number of physiological and pathological events in the CNS, as well as the role of neuroserpin as the natural regulator of tPA's activity in these processes. Increased or decreased activity of tPA leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, as a cytokine, tPA plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as a fibrogenic cytokine, it promotes the progression of kidney diseases.
Synonym: T-PA; TPA; t-plasminogen activator; Tissue plasminogen activator;
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分子量
- 28 kDa
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NCBI登录号
- NP_000921
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途径
- Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome
抗原
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