Nicastrin Protein (NCSTN) (Fc Tag)
Quick Overview for Nicastrin Protein (NCSTN) (Fc Tag) (ABIN7320109)
抗原
See all Nicastrin (NCSTN) 蛋白蛋白类型
宿主
资源
纯度
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标记
- This Nicastrin protein is labelled with Fc Tag.
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原理
- Recombinant Mouse Nicastrin/NCSTN Protein (Fc Tag)
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序列
- Met1-Glu668
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产品特性
- A DNA sequence encoding the mouse NCSTN (P57716) (Met1-Glu668) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Nicastrin (NCSTN) (AA 1-689) protein (GST tag)
NCSTN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nicastrin (NCSTN) protein (His tag)NCSTN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 99 % as determined by SDS-PAGE
Nicastrin (NCSTN) protein (Myc-DYKDDDDK Tag)NCSTN 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Nicastrin (NCSTN)
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别名
- Nicastrin/NCSTN
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背景
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Background: Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.
Synonym: 9430068N19Rik,AA727311,Aph2,D1Dau13e,Kiaa0253,mKIAA0253,NCSTN,Nct
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分子量
- 98.5 kDa
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UniProt
- P57716
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途径
- Notch Signaling, Neurotrophin Signaling Pathway
抗原
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