Nicastrin Protein (NCSTN) (His tag)
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- 抗原 See all Nicastrin (NCSTN) 蛋白
- Nicastrin (NCSTN)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Nicastrin protein is labelled with His tag.
- 原理
- Recombinant Human Nicastrin/NCSTN Protein (His Tag)
- 序列
- Met 1-Glu 669
- 产品特性
- A DNA sequence encoding the human NCSTN (NP_056146.1) extracellular domain (Met 1-Glu 669) was expressed, with a polyhistidine tag at the C-terminus.
- 纯度
- > 93 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product NCSTN 蛋白
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Nicastrin (NCSTN) (AA 1-689) protein (GST tag)
NCSTN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nicastrin (NCSTN) (AA 16-115) protein (GST tag)NCSTN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nicastrin (NCSTN) protein (His tag)NCSTN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 99 % as determined by SDS-PAGE
Nicastrin (NCSTN) protein (Fc Tag)NCSTN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Nicastrin (NCSTN) (AA 34-669) protein (His tag)Crystallography grade NCSTN 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Nicastrin (NCSTN) (AA 28-668) protein (His tag)Crystallography grade NCSTN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Nicastrin (NCSTN) (AA 34-709) protein (rho-1D4 tag)Crystallography grade NCSTN 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Nicastrin (NCSTN) (AA 28-708) protein (rho-1D4 tag)Crystallography grade NCSTN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Nicastrin (NCSTN)
- 别名
- Nicastrin/NCSTN (NCSTN 产品)
- 别名
- CG7012 Protein, Dmel\\CG7012 Protein, Nct Protein, Nic Protein, S5A Protein, ST96B Protein, agro Protein, dNct Protein, nic Protein, NCSTN Protein, DKFZp469O2418 Protein, APH2 Protein, 9430068N19Rik Protein, AA727311 Protein, Aph2 Protein, D1Dau13e Protein, Kiaa0253 Protein, mKIAA0253 Protein, nicastrin Protein, Nicastrin Protein, NCSTN Protein, nct Protein, ncstn Protein, CpipJ_CPIJ001617 Protein, Ncstn Protein, aph-2 Protein
- 背景
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Background: Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.
Synonym: APH2,ATAG1874,KIAA0253,NCSTN,RP11-517F10.1
- 分子量
- 72.4 kDa
- NCBI登录号
- NP_056146
- 途径
- Notch Signaling, Neurotrophin Signaling Pathway
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