MAPKAP Kinase 5 Protein (His-GST)
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Quick Overview for MAPKAP Kinase 5 Protein (His-GST) (ABIN7317049)
抗原
See all MAPKAP Kinase 5 (MAPKAPK5) 蛋白蛋白类型
宿主
资源
纯度
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标记
- This MAPKAP Kinase 5 protein is labelled with His-GST.
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原理
- Recombinant Human MAPKAPK5 Protein (His & GST Tag)
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序列
- Met 1-Gln 471
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产品特性
- A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 116,946.14 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-473) protein (His tag)
custom-made MAPKAPK5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-473) protein (His tag)custom-made MAPKAPK5 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-471) protein (GST tag)MAPKAPK5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA, KAA
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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别名
- MAPKAPK5
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背景
- MAPKAP-K5,MK-5,MK5,PRAK,MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.
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分子量
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Calculated MW: 82.0 kDa
Observed MW: 75 kDa
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基因ID
- 8550
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NCBI登录号
- NP_003659
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UniProt
- Q8IW41
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途径
- MAPK Pathway
抗原
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