MAP2K6 Protein (His-GST)
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Quick Overview for MAP2K6 Protein (His-GST) (ABIN7317108)
抗原
See all MAP2K6 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This MAP2K6 protein is labelled with His-GST.
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原理
- Recombinant Human MKK6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST Tag)
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序列
- Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp
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产品特性
- A DNA sequence encoding the human MAP2K6 isoform 1 (P52564-1) (Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 1250 nmol/min/mg using inactive MAPK14 as substrate.
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Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6) (AA 6-334) protein (His tag)
MAP2K6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6) protein (His-GST)MAP2K6 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 80 % as determined by reducing SDS-PAGE.
Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6) (AA 1-334) protein (GST tag)MAP2K6 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
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别名
- MKK6
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背景
- MAP2K6 ,MAPKK6,MEK6,MKK6,PRKMK6,SAPKK-3,SAPKK3,Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.
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分子量
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Calculated MW: 65.3 kDa
Observed MW: 60 kDa
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基因ID
- 5608
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UniProt
- P52564
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途径
- MAPK Pathway, TLR signaling, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades
抗原
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