c-MET Protein (AA 956-1390) (His-GST)
Our Local Distributor
北京 101111
Quick Overview for c-MET Protein (AA 956-1390) (His-GST) (ABIN7317093)
抗原
See all c-MET (MET) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 956-1390
-
标记
- This c-MET protein is labelled with His-GST.
-
原理
- Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)
-
序列
- Lys956-Ser1390
-
产品特性
- A DNA sequence encoding the human MET (P08581-1) (Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- 1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate. 2. Immobilized human HGFR (aa 956-1390) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/ml.
-
-
Met Proto-Oncogene (MET) (AA 25-932) protein (His-Avi Tag)
MET 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
Met Proto-Oncogene (MET) (AA 25-930) (Active) protein (His tag)MET 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Met Proto-Oncogene (MET) (AA 25-932) (Active) protein (Biotin,His-Avi Tag)MET 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Met Proto-Oncogene (MET) (AA 25-932) (Active) protein (His tag,AVI tag,Biotin)MET 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Met Proto-Oncogene (MET) (AA 26-125) protein (GST tag)MET 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Met Proto-Oncogene (MET) (AA 956-1080) protein (His-IF2DI Tag)MET 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Met Proto-Oncogene (MET) (AA 25-932) protein (His tag)MET 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Met Proto-Oncogene (MET) (AA 25-932) protein (Fc Tag)MET 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Met Proto-Oncogene (MET) (Active) protein (His tag)MET 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. ELISA Active
Met Proto-Oncogene (MET) (Active) protein (Fc Tag)MET 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 92 % as determined by reducing SDS-PAGE. ELISA Active
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 3 mM DTT
-
储存液
- Dithiothreitol (DTT)
-
注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
-
有效期
- 6 months
-
-
- c-MET (MET) (Met Proto-Oncogene (MET))
-
别名
- c-MET/HGFR
-
背景
- AUTS9,DFNB97,HGFR,RCCP2,c-Met,Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
-
分子量
-
Calculated MW: 76.8 kDa
Observed MW: 68 kDa
-
基因ID
- 4233
-
UniProt
- P08581
-
途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原
-
