Insulin Receptor Protein (INSR) (His-GST)
This Recombinant Insulin Receptor protein is expressed in Baculovirus infected Insect Cells.
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产品编号 ABIN7317078
发货至:
中国
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Quick Overview for Insulin Receptor Protein (INSR) (His-GST) (ABIN7317078)
抗原
See all Insulin Receptor (INSR) 蛋白
Insulin Receptor (INSR)
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
Baculovirus infected Insect Cells
纯度
> 92 % as determined by reducing SDS-PAGE.
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标记
- This Insulin Receptor protein is labelled with His-GST.
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原理
- Recombinant Human Insulin Receptor/INSR Protein (His & GST Tag)
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序列
- Gly 989-Ser 1382
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产品特性
- A DNA sequence encoding the human INSR isoform long (NP_000199.2) cytoplasmic domain (Gly 989-Ser 1382) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 45 nmol/min/mg using Poly(Ala, Glu, Lys, Tyr)6:2:5:1 as substrate.
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Insulin Receptor (INSR) (AA 28-944) (Active) protein (His tag)
INSR 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Insulin Receptor (INSR) (AA 881-980) protein (GST tag)INSR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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缓冲液
- Supplied as sterile 50 mM Tris, 100 mM NaCI, pH 7.5, 25 % glycerol, 1 mM TCEP,0.5 mM GSH
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- Insulin Receptor (INSR)
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别名
- Insulin Receptor/INSR
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背景
- CD220,HHF5,Insulin Receptor,INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).
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分子量
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Calculated MW: 72.3 kDa
Observed MW: 70 kDa
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基因ID
- 3643
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NCBI登录号
- NP_000199
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UniProt
- P06213
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途径
- NF-kappaB Signaling, RTK signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity
抗原
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