GAD65 Protein (His-GST)
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Quick Overview for GAD65 Protein (His-GST) (ABIN7320201)
抗原
See all GAD65 (GAD2) 蛋白蛋白类型
宿主
资源
纯度
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标记
- This GAD65 protein is labelled with His-GST.
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原理
- Recombinant Mouse GAD65/GAD2/GAD-2 Protein (His & GST Tag)
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序列
- Met 1-Leu 585
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产品特性
- A DNA sequence encoding full length of mouse GAD2 (NP_032104.2) (Met 1-Leu 585) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) protein (GST tag)
GAD2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) proteinGAD2 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) protein (GST tag)GAD2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585), (full length) protein (His tag)GAD2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GAD2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM GSH, 3 mM DTT, 10 % glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存液
- Dithiothreitol (DTT), PMSF
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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别名
- GAD65/GAD2/GAD-2
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背景
- 6330404F12Rik,GAD(65),GAD65,Gad-2,Glutamate decarboxylase 2, also known as glutamate decarboxylase 65 kDa isoform, 65 kDa glutamic acid decarboxylase, GAD2 and GAD65, is a member of thegroup II decarboxylase family. GAD2 is identified as a major autoantigen in insulin-dependent diabetes. GAD2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. GAD2 is implicated in the formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in the regulation of food intake. GABA is synthesized in brain by two isoforms of glutamic acid decarboxylase (Gad), GAD1 and GAD2. GAD1 provides most of the GABA in brain, but GAD2 can be rapidly activated in times of high GABA demand. Mice lacking GAD2 are viable whereas deletion of GAD1 is lethal. Deletion of GAD2 increased ethanol palatability and intake and slightly reduced the severity of ethanol-induced withdrawal.
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分子量
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Calculated MW: 93.0 kDa
Observed MW: 80 kDa
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NCBI登录号
- NP_032104
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UniProt
- P48320
抗原
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