C5A 蛋白
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- 抗原 See all C5A (C5a) 蛋白
- C5A (C5a) (Complement Component 5a (C5a))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 原理
- Recombinant Human Complement C5a Protein (Active)
- 序列
- Leu 679-Arg 751
- 产品特性
- A DNA sequence encoding the active form of human C5a (Leu 679-Arg 751) was expressed.
- 纯度
- > 94 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is typically 5-15 ng/ml.
- Top Product
- Discover our top product C5a 蛋白
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Complement Component 5a (C5a) (AA 678-751) protein (His tag)
C5a 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Complement Component 5a (C5a) (AA 1-77) protein (His tag)C5a 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Complement Component 5a (C5a) (AA 679-751) proteinC5a 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE.
Complement Component 5a (C5a) (AA 678-751) (Active) proteinC5a 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
Complement Component 5a (C5a) (AA 679-755) (Active) protein (His tag)C5a 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
Complement Component 5a (C5a) (AA 679-751) (Active) protein (Fc Tag)C5a 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- C5A (C5a) (Complement Component 5a (C5a))
- 别名
- Complement C5a (C5a 产品)
- 别名
- C5a Protein, C5b Protein, CPAMD4 Protein, Hc Protein, RGD1561905 Protein, complement C5 Protein, C5 Protein
- 背景
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Background: C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.
Synonym: Complement C5; C5a anaphylatoxin; C5a;CPAMD4;ECLZB;C5Da
- 分子量
- 8.3 kDa
- NCBI登录号
- NP_001726
- 途径
- Complement System, Carbohydrate Homeostasis
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