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M-CSF/CSF1 Protein (AA 33-262)

This Recombinant M-CSF/CSF1 protein is expressed in HEK-293 Cells.

ELISA

Western Blotting (WB) image for Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-262) (Active) protein (ABIN7320618) (M-CSF/CSF1 Protein (AA 33-262))

2 images

产品编号 ABIN7320618

发货至: 中国

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Quick Overview for M-CSF/CSF1 Protein (AA 33-262) (ABIN7320618)

抗原

See all M-CSF/CSF1 (CSF1) 蛋白

M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

蛋白类型

Recombinant

生物活性

Active

宿主

小鼠

资源

HEK-293 Cells

应用范围

Cell Culture (CC)

纯度

> 95 % as determined by reducing SDS-PAGE.

Discover our top product CSF1 蛋白

产品特性

AA 33-262

原理

Recombinant Mouse M-CSF/CSF1 Protein

序列

Lys33-Glu262

产品特性

Recombinant Mouse Macrophage colony-stimulating factor 1 is produced by our Mammalian expression system and the target gene encoding Lys33-Glu262 is expressed.

过滤

0.2 μm filtered

内毒素水平

< 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.04-0.2 ng/ml.
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Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) (Active) protein
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (Fc Tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) (Active) protein (His-Avi Tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (His tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) (Active) protein (His tag)
CSF1 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) (Active) protein (Biotin,His-Avi Tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190), (Isoform 3) (Active) protein
CSF1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as analyzed by non-reducing SDS-PAGE. Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (mFc Tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-187) (Active) protein
CSF1 宿主: 小鼠宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) (Active) protein (His tag,AVI tag,Biotin)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Lyophilized

缓冲液

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

储存条件

4 °C,-20 °C,-80 °C

储存方法

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

有效期

12 months
抗原

M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

别名

M-CSF/CSF1

背景

CSF-1,Csf1,Csfm,MCSF,Macrophage colony-stimulating factor 1,Macrophage colony-stimulating factor 1(M-csf)is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.

分子量

Calculated MW: 26.0 kDa
Observed MW: 37-80 kDa

UniProt

P07141

途径

RTK signaling