CD40 Ligand Protein (CD40LG) (AA 108-261) (His tag)
Our Local Distributor
北京 101111
Quick Overview for CD40 Ligand Protein (CD40LG) (AA 108-261) (His tag) (ABIN7319661)
抗原
See all CD40 Ligand (CD40LG) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 108-261
-
标记
- This CD40 Ligand protein is labelled with His tag.
-
原理
- Recombinant Human CD40L/TNFSF5 Protein (His Tag)
-
序列
- Glu108-Leu261
-
产品特性
- Recombinant Human CD40L is produced by our Mammalian expression system and the target gene encoding Glu108-Leu261 is expressed with a 6His tag at the C-terminus.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Loaded Human CD40-Fc(PKSH032216) on Protein A Biosensor, can bind Human CD40L-His(PKSH033726) with an affinity constant of 2.74 nM as determined in BLI assay.
-
-
想要此Protein的其他选项吗？
!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
-
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) (Active) protein (Fc Tag)
CD40LG 宿主: 人, 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) (Active) protein (Biotin,His-DYKDDDDK Tag)CD40LG 宿主: 人, 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) (Active) protein (His-DYKDDDDK Tag)CD40LG 宿主: 人, 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD40 Ligand (CD40LG) (AA 116-261) protein (His-DYKDDDDK-Strep II Tag)CD40LG 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
CD40 Ligand (CD40LG) (AA 112-260), (Trimer) (Active) protein (Fc Tag)CD40LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG 宿主: 人, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 1-261) protein (GST tag)CD40LG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD40 Ligand (CD40LG) (AA 99-330) protein (Fc Tag)CD40LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from a 0.2 μm filtered solution of PBS, 1 mM EDTA, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- CD40 Ligand (CD40LG)
-
别名
- CD40L/TNFSF5
-
背景
- CD154,CD40 Ligand,CD40-L,CD40L,CD40LG,HIGM1,IGM,IMD3,T-BAM,T-Cell Antigen Gp39,TNF-Related Activation Protein,TNFSF5,TRAP,Tumor Necrosis Factor Ligand Superfamily Member 5,hCD40L,CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40, its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
-
分子量
-
Calculated MW: 19.1 kDa
Observed MW: 19-22 kDa
-
基因ID
- 959
-
UniProt
- P29965
-
途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
-
