电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

CSK 蛋白

This Recombinant CSK protein is expressed in Baculovirus infected Insect Cells.
产品编号 ABIN7320121
发货至: 中国

Quick Overview for CSK 蛋白 (ABIN7320121)

抗原

See all CSK 蛋白
CSK (C-Src tyrosine Kinase (CSK))

蛋白类型

Recombinant

宿主

  • 8
  • 1
  • 1
  • 1
  • 1
小鼠

资源

  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Baculovirus infected Insect Cells

纯度

> 90 % as determined by SDS-PAGE
  • 原理

    Recombinant Mouse CSK/C-Src kinase Protein (Active)

    序列

    Met 1-Leu 450

    产品特性

    A DNA sequence encoding the mouse CSK (P41241?) (Met 1-Leu 450) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.

    内毒素水平

    < 1.0 EU per μg of the protein as determined by the LAL method.

    Biological Activity Comment

    Kinase activity untested
  • 想要此Protein的其他选项吗?

    !
    探索我们的预定义定制蛋白和定制蛋白服务!

    您的项目需要进一步定制吗?联系我们,了解我们的定制蛋白解决方案

  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Please refer to the printed manual for detailed information.

    缓冲液

    Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • 抗原

    CSK (C-Src tyrosine Kinase (CSK))

    别名

    CSK/C-Src kinase

    背景

    Background: The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development. References

    Synonym: AW212630,p50CSK

    分子量

    50.9 kDa

    UniProt

    P41241

    途径

    TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
You are here:
Chat with us!