CSK Protein (His-GST)
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北京 101111
Quick Overview for CSK Protein (His-GST) (ABIN7320001)
抗原
See all CSK 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This CSK protein is labelled with His-GST.
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原理
- Recombinant Mouse CSK/C-Src kinase Protein (His & GST Tag)
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序列
- Met 1-Leu 450
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产品特性
- A DNA sequence encoding the mouse CSK (P41241?) (Met 1-Leu 450) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 70 nmol/min/mg using Poly(Glu, Tyr) 4:1 as substrate.
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C-Src tyrosine Kinase (CSK) (AA 1-450) protein (GST tag)
CSK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
C-Src tyrosine Kinase (CSK) (AA 1-450) protein (His tag)CSK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
C-Src tyrosine Kinase (CSK) (Active) proteinCSK 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
C-Src tyrosine Kinase (CSK) (Active) protein (GST tag)CSK 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 92 % as determined by reducing SDS-PAGE. Active
C-Src tyrosine Kinase (CSK) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)CSK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- CSK (C-Src tyrosine Kinase (CSK))
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别名
- CSK/C-Src kinase
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背景
- AW212630,p50CSK,The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tail. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development.
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分子量
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Calculated MW: 78.5 kDa
Observed MW: 65 kDa
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途径
- TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
抗原
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