Aminoacylase 1 Protein (ACY1) (His tag)
Quick Overview for Aminoacylase 1 Protein (ACY1) (His tag) (ABIN7317832)
抗原
See all Aminoacylase 1 (ACY1) 蛋白蛋白类型
宿主
资源
纯度
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标记
- This Aminoacylase 1 protein is labelled with His tag.
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原理
- Recombinant Human ACY1/Aminoacylase-1 Protein (His Tag)
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序列
- Met 1-Ser 408
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产品特性
- A DNA sequence encoding the full length of human ACY1 (NP_000657.1) (Met 1-Ser 408) was expressed with a polyhistidine tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Aminoacylase 1 (ACY1) (AA 1-408) protein (His tag)
ACY1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Aminoacylase 1 (ACY1) (AA 1-408) protein (His tag)ACY1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Aminoacylase 1 (ACY1) (AA 1-408) protein (GST tag)ACY1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Aminoacylase 1 (ACY1) (Active) protein (His tag)ACY1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Aminoacylase 1 (ACY1) protein (DYKDDDDK Tag)ACY1 宿主: 人 宿主: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Aminoacylase 1 (ACY1) protein (Myc-DYKDDDDK Tag)ACY1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Aminoacylase 1 (ACY1)
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别名
- ACY1/Aminoacylase-1
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背景
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Background: Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
Synonym: ACY-1,ACY1D,HEL-S-5
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分子量
- 47.3 kDa
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NCBI登录号
- NP_000657
抗原
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