ADK Protein (His-GST)
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北京 101111
Quick Overview for ADK Protein (His-GST) (ABIN7194156)
抗原
See all ADK 蛋白蛋白类型
宿主
资源
纯度
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标记
- This ADK protein is labelled with His-GST.
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原理
- Recombinant Human ADK Protein (His & GST Tag)
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序列
- Met 1-His 345
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产品特性
- A DNA sequence encoding the human ADK isoform short (AAH03568.1) (Met 1-His 345) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Adenosine Kinase (ADK) (AA 1-361) protein (His tag)
custom-made ADK 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adenosine Kinase (ADK) (AA 1-345) protein (GST tag)ADK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adenosine Kinase (ADK) (AA 22-362) protein (His tag)ADK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Adenosine Kinase (ADK) (AA 22-362) (Active) protein (His tag)ADK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, EAA Active
Adenosine Kinase (ADK) (AA 1-361) (Active) protein (His tag)ADK 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, EAA Active
Adenosine Kinase (ADK) (AA 22-362) protein (His-IF2DI Tag)ADK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 0.3 mM DTT
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- ADK (Adenosine Kinase (ADK))
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别名
- ADK
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背景
- AK,Adenosine kinase(ADK) belongs to the family of transferases. Adenosine kinase (ADK) is the key enzyme in adenosine metabolism and catalyzes ATP and adenosine into two products: ADP and AMP. Two isoforms of the enzyme adenosine kinase (ADK), which differ at their N-terminal ends, are found in mammalian cells. It has been shown that the two ADK isoforms differ only in their first exons and the promoter regions, hence they arise via differential splicing of their first exons with the other exons common to both isoforms. In adult brain, ADK is primarily present in astrocytes. Several lines of experimental evidence support a critical role of ADK in different types of brain injury associated with astrogliosis, which is also a prominent morphologic feature of temporal lobe epilepsy (TLE). It has been suggested that dysregulation of ADK in astrocytes is a common pathologic hallmark of TLE. Moreover, in vitro data suggest the existence of an additional layer of modulatory crosstalk between the astrocyte-based adenosine cycle and inflammation. ADK also contributes to CK homeostasis in vivo.
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分子量
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Calculated MW: 68.0 kDa
Observed MW: 60 kDa
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基因ID
- 132
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UniProt
- P55263
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途径
- Ribonucleoside Biosynthetic Process
抗原
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