ADK Protein (AA 22-362) (His tag)
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Quick Overview for ADK Protein (AA 22-362) (His tag) (ABIN6388145)
抗原
See all ADK 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 22-362
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标记
- This ADK protein is labelled with His tag.
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序列
- MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH
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纯化方法
- > 95% by SDS-PAGE
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Biological Activity Comment
- Specific activity is > 30pmol/min/ug and is defined as the amount of enzyme that convert 1.0pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.
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Adenosine Kinase (ADK) (AA 1-361) protein (His tag)
custom-made ADK 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adenosine Kinase (ADK) (AA 22-362) protein (His-IF2DI Tag)ADK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Adenosine Kinase (ADK) protein (GST tag,His tag)ADK 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Adenosine Kinase (ADK) (AA 1-345) protein (GST tag)ADK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adenosine Kinase (ADK) (AA 1-361) (Active) protein (His tag)ADK 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, EAA Active
Adenosine Kinase (ADK) (AA 22-362) protein (His tag)ADK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 20 % glycerol, 1 mM EDTA, 50 mM NaCl
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ADK (Adenosine Kinase (ADK))
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别名
- Adenosine Kinase/ADK
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背景
- Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 40.5 kDa (362aa ) confirmed by MALDI-TOF
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NCBI登录号
- NP_006712
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途径
- Ribonucleoside Biosynthetic Process
抗原
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