Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-0.7 ng/mL.
IFNG
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE.
WB, ELISA
Active
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 5 % Sucrose, 4 % Mannitol, 0.02 % Tween 80, pH 7.4.
储存条件
-20 °C,-80 °C
储存方法
The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.