Quick Overview for BAFF 蛋白 (ABIN6700762)
抗原
See all BAFF (TNFSF13B) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-u65-0020
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Supplier
- Rockland
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原理
- Human BAFF
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纯化方法
- BAFF purity was determined to be greater than 90% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by a RPMI-8226 cell survival assay in the presence of 0.2 μM DEX and is typically less than 30 ng/mL.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 136,947.45 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 172,528.32 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 141-285), (Trimer) protein (His-DYKDDDDK Tag)
TNFSF13B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) protein (Fc Tag)TNFSF13B 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 134-285) protein (Fc Tag)TNFSF13B 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 141-285), (Trimer) protein (hFc-DYKDDDDK Tag)TNFSF13B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 94 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (Trimer) protein (His-Avi Tag,Biotin)TNFSF13B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 94% as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 141-285), (Trimer) protein (His-Avi Tag,DYKDDDDK Tag,Biotin)TNFSF13B 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 1-285) protein (His tag)custom-made TNFSF13B 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 1-309) protein (His tag)custom-made TNFSF13B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 141-285), (Trimer) protein (His-Avi Tag)TNFSF13B 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 136-285) protein (His-DYKDDDDK-Strep II Tag)TNFSF13B 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
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应用备注
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Application Note: Human BAFF Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-BAFF in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
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别名
- TNFSF13B
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背景
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Synonyms: THANK, B lymphocyte stimulator (BLyS), B-cell activating factor, TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1), CD_antigen=CD257
Background: Human B cell Activating Factor (BAFF), is a Type II member of the TNF superfamily. Although it is typically a transmembrane protein expressed on T cells, macrophages and dendritic cells, it can also be cleaved in the extracellular region to produce a soluble form detectable in the serum. BAFF is thought to be important for the survival and maturation of peripheral B cells. BAFF initiates signaling through three receptors with human BAFF having activity on mouse splenocytes. Recombinant human BAFF is a non-glycosylated protein containing a N terminal His tag, a thrombin cut site and 153 amino acids of the TNF-like domain. This protein has a total molecular weight of 18.5 kDa.
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UniProt
- Q9Y275
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途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response
抗原
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