Quick Overview for RANKL 蛋白 (ABIN6700760)
抗原
See all RANKL (TNFSF11) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-c22
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Supplier
- Rockland
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原理
- RANKL Mouse Recombinant Protein
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纯化方法
- Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
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内毒素水平
- Measured by LAL is 0.148 EU/μg protein.
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Biological Activity Comment
- Mouse RANKL has full biological activity when compared to a standard. The ED50, as measured by the dose-dependent induction of TNF-a production in RAW 264.7 cells, is 50 ng/ml.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 63-244) protein (His-DYKDDDDK Tag)
TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 63-244) proteinTNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 1-316) protein (His tag)custom-made TNFSF11 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 1-317) protein (His tag)custom-made TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 70-316) protein (His tag)TNFSF11 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 158-317) protein (His-DYKDDDDK-Strep II Tag)TNFSF11 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (His tag)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) protein (Fc Tag)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (AVI tag,Fc Tag,Biotin)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 64-245) (Active) protein (His tag,AVI tag,Biotin)TNFSF11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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应用备注
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Application Note: RANKL protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-RANKL in immunological assays. Formulation of buffer: 10 mM Sodium Phosphate, 20 mM Sodium Chloride, pH : 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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浓度
- 0.1 mg/mL
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缓冲液
- Formulation of buffer: 10 mM Sodium Phosphate, 20 mM Sodium Chloride, pH : 7.5.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
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别名
- Tnfsf11
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背景
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Synonyms: TNF-related activation-induced cytokine, TRANCE, Tumor necrosis factor ligand superfamily member 11, TNFRSF11, Osteoprotegerin ligand, OPG, Osteoclast differentiation factor, ODF, CD254
Background: The secreted cytokine RANKL (Receptor Activator of Nuclear factor kappa-B Ligand) is critically involved in osteoclastic differentiation and activation and in the regulation of specific immunity. RANKL exists as a homotrimer, is glycosylated, and occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL (sRANKL), which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANK Ligand, which is enzymatically processed by TACE (TNF-alpha converting enzyme (TACE, ADAM-17)). All three forms stimulate their specific receptor, RANK, which is located on osteoclastic and dendritic cells. RANKL binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. It may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Deficiency in Tnfsf11 results in failure to form lobulo-alveolar mammary structures during pregnancy, resulting in death of newborns. Trance-deficient mice show severe osteopetrosis. RANKL is highly expressed in thymus and lymph nodes, but not in non-lymphoid tissues and is abundantly expressed in T-cells but not in B-cells. A high level expression is also seen in the trabecular bone and lung. Recombinant Mouse soluble RANKL is approximately 19.9 kDa and contains 174 amino acids.
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UniProt
- O35235
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途径
- NF-kappaB Signaling
抗原
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