Galectin 3 Protein (LGALS3)
Quick Overview for Galectin 3 Protein (LGALS3) (ABIN6700206)
抗原
See all Galectin 3 (LGALS3) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-u92-0010
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Supplier
- Rockland
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原理
- Human Galectin-3 Recombinant Protein
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纯化方法
- Galectin-3 purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL.
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Galectin 3 (LGALS3) (AA 2-250) protein (His tag)
LGALS3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Galectin 3 (LGALS3) (AA 2-264) protein (His tag)LGALS3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Galectin 3 (LGALS3) (AA 2-262) protein (His tag)LGALS3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Galectin 3 (LGALS3) (AA 4-262) protein (His tag)LGALS3 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Galectin 3 (LGALS3) (AA 1-264) protein (His tag)LGALS3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Galectin 3 (LGALS3) (AA 2-250) protein (His tag)LGALS3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Galectin 3 (LGALS3) (AA 1-264) (Active) protein (His tag)LGALS3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS Active
Galectin 3 (LGALS3) (AA 1-250) protein (GST tag)LGALS3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Galectin 3 (LGALS3) proteinLGALS3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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应用备注
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Application Note: Galectin-3 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Galectin-3 in immunological assays. Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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缓冲液
- Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- Galectin 3 (LGALS3)
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别名
- LGALS3
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背景
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Synonyms: 35 kDa lectin, Galactose-specific lectin-3, Galactoside-binding protein (GALBP), IgE-binding protein, Laminin-binding protein, MAC2, L-29 CPB-35
Background: Galectin-3 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-3 is expressed by a wide range of cell types including activated T cells, tumor cells, macrophages, osteoclasts, fibroblasts and epithelial cells and interacts with β-galactoside sugar moieties. Galectin-3 is associated with cancer, heart failure, stroke and inflammation. Human and mouse Galectin-3 share an 80 % homology by amino acid sequence. Recombinant human Galectin-3 is a non-glycosylated protein, containing 250 amino acids, with a molecular weight of 26 kDa.
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UniProt
- P17931
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途径
- RTK signaling
抗原
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