KIT Ligand Protein (KITLG)
(1 reference)
Quick Overview for KIT Ligand Protein (KITLG) (ABIN6700197)
抗原
See all KIT Ligand (KITLG) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-w20-0100
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Supplier
- Rockland
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原理
- Mouse Stem Cell Factor Recombinant Protein
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纯化方法
- Stem Cell Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of human TF-1 cells and is typically less than 10 ng/mL.
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KIT Ligand (KITLG) (Active) protein
KITLG 宿主: 人 宿主: Yeast (Pichia pastoris) > 95 % by SDS-PAGE Active
KIT Ligand (KITLG) (AA 26-214) protein (Fc Tag)KITLG 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-189) protein (His tag)KITLG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-190) protein (His tag)KITLG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-189) protein (His-IF2DI Tag)KITLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-189) (Active) proteinKITLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
KIT Ligand (KITLG) (AA 1-245) protein (GST tag)KITLG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
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Application Note: Stem Cell Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Stem Cell Factor in immunological assays. Lyophilized with 20 mM sodium phosphate, pH 6.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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浓度
- 0.1 mg/mL
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缓冲液
- Lyophilized with 20 mM sodium phosphate, pH 6.5.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).
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: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).
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- KIT Ligand (KITLG)
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别名
- Kitlg
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背景
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Synonyms: c-Kit Ligand, Hematopoietic growth factor KL, KL, Steel Factor, Stem cell factor (SCF), Mast cell growth factor(MGF)
Background: Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant mouse SCF is a non-glycosylated protein, containing 164 amino acids, with a molecular weight of 18.3 kDa.
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UniProt
- P20826
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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