IL-5 蛋白
Quick Overview for IL-5 蛋白 (ABIN6700150)
抗原
See all IL-5 (IL5) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-v42-0100
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Supplier
- Rockland
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原理
- Mouse Interleukin-5 Recombinant Protein
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纯化方法
- Interleukin-5 purity was determined to be greater than 97% as determined by HpLC and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent induction of TF-1 cell proliferation and is typically less than 2 ng/mL.
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Interleukin 5 (IL5) (AA 20-134) protein (His-Avi Tag)
IL5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-134) protein (His-Avi Tag,Biotin)IL5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 21-133) protein (His-Avi Tag)IL5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 5 (IL5) (AA 20-134) protein (His tag)IL5 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 21-133) protein (His-Avi Tag,Biotin)IL5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-134) protein (His tag)IL5 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 5 (IL5) (AA 20-134) protein (Fc Tag)IL5 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 5 (IL5) protein (mFc Tag)IL5 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 5 (IL5) protein (His tag)IL5 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 5 (IL5) (AA 20-132) protein (His tag)IL5 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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应用备注
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Application Note: Interleukin-5 Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Interleukin-5 in immunological assays. Lyophilized in 20 mM sodium bicarbonate, pH 9.0.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
- Lyophilized in 20 mM sodium bicarbonate, pH 9.0.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL-5 (IL5) (Interleukin 5 (IL5))
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别名
- Il5
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背景
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Synonyms: EDF, B-cell growthfactor II (BCDFII), Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor (TRF)
Background: Interleukin-5 (IL-5) is a hematopoietic growth factor expressed in Th2, mast cells and eosinophils. IL-5 acts through its receptor, the IL-5 receptor (IL-5R) and is involved in B cell growth and eosinophil activation. IL-5 has been shown to be and is regulated by GATA-3, in addition to other transcription factors. Human and mouse IL-5 are cross-reactive. Recombinant mouse IL-5 is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains, with a total molecular weight of 26.2 kDa.
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UniProt
- P04401
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
抗原
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