IL-3 蛋白
Quick Overview for IL-3 蛋白 (ABIN6700118)
抗原
See all IL-3 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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Supplier Product No.
- 010-f01-b92-0010
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Supplier
- Rockland
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原理
- Mouse Interleukin-3 Recombinant Protein (Animal Free)
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纯化方法
- Interleukin-3 is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 97% as determined by HPLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-PAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent stimulation of mouse M-NFS-60 cells and is typically less than 0.05 ng/mL.
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 27-166) protein (His-IF2DI Tag)IL-3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 3 (IL-3) (AA 33-166) (Active) protein (His tag)IL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
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Application Note: Mouse Interleukin-3 Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Mouse Interleukin-3 in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL-3 (Interleukin 3 (IL-3))
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别名
- Il3
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背景
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Synonyms: Mast cell growth factor (MCGF), Multipotential colony-stimulating factor, Multi-CSF, Hematopoietic growth factor, HCGF, P-cell stimulation factor
Background: Interleukin-3 (IL-3) is a cytokine produced by activated T cells and mast cells. IL-3 is able to induce the differentiation of hematopoietic stem cells to precursor cells of myeloid lineage (erythrocytes, megakaryocytes, granulocytes, dendritic cells and monocytes). IL-3 also has functions in the nervous system and appears to be important in several chronic inflammatory diseases. Recombinant mouse IL-3 is a non-glycosylated protein, containing 135 amino acids, with a molecular weight of 15.1 kDa.
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UniProt
- P01586
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途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原
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