Quick Overview for IL-3 蛋白 (ABIN6700110)
抗原
See all IL-3 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-b92-0.1
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Supplier
- Rockland
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原理
- IL-3 Recombinant Human Protein
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纯化方法
- Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.
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内毒素水平
- Measured by LAL is < 1 EU/μg protein.
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Biological Activity Comment
- Human IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 2 ng/ml.
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (Fc Tag)IL-3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 3 (IL-3) (AA 20-152) (Active) proteinIL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
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应用备注
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Application Note: IL-3 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-3 in immunological assays. Buffer formation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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浓度
- 0.1 mg/mL
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缓冲液
- Buffer formation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL-3 (Interleukin 3 (IL-3))
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别名
- IL3
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背景
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Synonyms: Interleukin-3 cytokine, IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, Mast cell growth factor, MCGF
Background: Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases. Recombinant Human IL-3 produced in E. coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15,000 Daltons.
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UniProt
- P08700
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途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原
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