Quick Overview for IGF1 蛋白 (ABIN6699961)
抗原
See all IGF1 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-v21-0100
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Supplier
- Rockland
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原理
- Human Insulin-like Growth Factor I Recombinant Protein
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纯化方法
- Insulin-like Growth Factor I purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse FDC-P1 cells is typically less than 1.0 ng/mL.
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Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) (Active) protein
IGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His tag)IGF1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His-IF2DI Tag)IGF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) proteinIGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Insulin-Like Growth Factor 1 (IGF1) (AA 49-138) protein (GST tag)IGF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 宿主: 小鼠 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 (IGF1) (AA 1-153) protein (GST tag)IGF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
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Application Note: Insulin-like Growth Factor I Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Insulin-like Growth Factor I in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- IGF1
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背景
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Synonyms: Somatamedin C, mechano growth factor (MGF), IGF-IA
Background: Insulin-like Growth Factor I, IGF-I, is a growth factor produced by the liver when stimulated with growth hormone and can be found circulating throughout the body . IGF-I activates the IGF-I receptor (IGF1R) and the insulin receptor to mediate growth of almost every cell of the body. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Mature human IGF-I is 100 % homologous with bovine and porcine proteins. Recombinant human IGF-I is a non-glycosylated protein, containing 70 amino acids, with a molecular weight of 7.7 kDa.
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UniProt
- P05019
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途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
抗原
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