FGF4 蛋白
Quick Overview for FGF4 蛋白 (ABIN6699859)
抗原
See all FGF4 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-u76-0025
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Supplier
- Rockland
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原理
- Human Fibroblast Growth Factor-4 Recombinant Protein
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纯化方法
- Fibroblast Growth Factor-4 purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by its ability to induce the proliferation of mouse NR6R-3T3 fibroblasts and is typically 0.25-1.25 ng/mL.
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Fibroblast Growth Factor 4 (FGF4) protein
FGF4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Fibroblast Growth Factor 4 (FGF4) (AA 71-206) protein (His tag)FGF4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Fibroblast Growth Factor 4 (FGF4) (AA 25-206) protein (His tag)FGF4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 4 (FGF4) proteinFGF4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
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应用备注
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Application Note: Fibroblast Growth Factor-4 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor-4 in immunological assays. Lyophilized in 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 25 μL (25-250 μL)
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缓冲液
- Lyophilized in 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- FGF4 (Fibroblast Growth Factor 4 (FGF4))
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别名
- FGF4
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背景
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Synonyms: Transforming protein KS3, Heparin-binding growth factor (HBGF-4), Heparin secretory-transforming protein 1 (HST-1)
Background: Fibroblast Growth Factor 4 (FGF-4) is a growth factor predominantly expressed during embryonic development, playing a key role in limb development. In culture, FGF-4 has been shown to be an important regulator of growth for stem cells, fibroblasts and endothelial cells. Unglycosylated FGF-4 is N-terminally cleaved into 13 kDa or 15 kDa proteins that are more active than the precursor 19 kDa protein, Bellosa P, et al. (1993). Human FGF-4 shares high homology and cross-reactivity with the mouse protein. Recombinant human FGF-4 is a non-glycosylated protein containing 140 amino acids, with a total molecular weight of 15 kDa.
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UniProt
- P08620
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance
抗原
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