M-CSF/CSF1 蛋白
Quick Overview for M-CSF/CSF1 蛋白 (ABIN6699714)
抗原
See all M-CSF/CSF1 (CSF1) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-v78-0100
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Supplier
- Rockland
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原理
- Mouse Macrophage Colony Stimulating Factor Recombinant Protein
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纯化方法
- Macrophage Colony Stimulating Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by dose-dependent induction of M-NFS-60 cell proliferation and is typically less than 2 ng/mL.
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应用备注
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Application Note: Macrophage Colony Stimulating Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Macrophage Colony Stimulating Factor in immunological assays. Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
- Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
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别名
- Csf1
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背景
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Synonyms: MGI-IM
Background: Macrophage Colony Stimulating Factor (M-CSF) is hematopoietic growth factor produced by a wide variety of cells. M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells. Recombinant mouse M-CSF is a disulfide-linked homodimer, containing two 156 amino acid chains, with a total molecular weight of 36.8 kDa.
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UniProt
- P07141
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途径
- RTK signaling
抗原
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