Quick Overview for CCL3 蛋白 (ABIN6699626)
抗原
See all CCL3 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-315
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Supplier
- Rockland
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原理
- MIP-1α Human Recombinant Protein
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纯化方法
- Purity was determined to be greater than 97% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
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内毒素水平
- Measured by LAL is 0.15 EU/μg protein.
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Biological Activity Comment
- Determined by its ability to chemoattract human monocytes using a concentration range of 0.5 - 8 ng/ml.
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Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 1-92) protein (GST tag)
CCL3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) protein (His tag)CCL3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 23-92) protein (His tag)CCL3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) (Active) proteinCCL3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA Active
Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) protein (His tag)CCL3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) protein (His tag)CCL3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-C Motif) Ligand 3 (CCL3) protein (His tag)CCL3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Chemokine (C-C Motif) Ligand 3 (CCL3) proteinCCL3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 27-92) protein (Fc Tag)CCL3 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) (Active) protein (Biotin)CCL3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA Active
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应用备注
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Application Note: MIP-1α protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti--MIP-1α in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
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浓度
- 0.1 mg/mL
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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别名
- CCL3
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背景
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Synonyms: Human Macrophage Inflammatory Protein-1, CCL3 alpha cytokine, C-C motif chemokine 3, Small-inducible cytokine A3, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, SIS-beta, PAT 464.1
Background: Recombinant Human Macrophage Inflammatory Protein-1 (CCL3, MIP-1) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7820 Daltons.
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UniProt
- P10147
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途径
- Cellular Response to Molecule of Bacterial Origin, Autophagy
抗原
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