ENO1 Protein (AA 1-434) (His tag)
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- 抗原 See all ENO1 蛋白
- ENO1 (Enolase 1 (ENO1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-434
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ENO1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGS HAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSC NCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK
- 纯度
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.
- Top Product
- Discover our top product ENO1 蛋白
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Enolase 1 (ENO1) (AA 1-434) protein (GST tag)
ENO1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enolase 1 (ENO1) (AA 1-434) proteinENO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Enolase 1 (ENO1) protein (His tag)ENO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Enolase 1 (ENO1) (AA 2-433) protein (His tag)ENO1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Enolase 1 (ENO1) (AA 325-434) protein (GST tag)ENO1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enolase 1 (ENO1) (AA 1-434) (Active) proteinENO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS Active
Enolase 1 (ENO1) (AA 2-428), (partial) protein (GST tag)ENO1 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Enolase 1 (ENO1) (AA 2-433), (partial) protein (His tag)ENO1 宿主: 小鼠 宿主: 酵母菌 Recombinant > 90 % ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.5) containing 40 % glycerol, 0.1M NaCl
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- ENO1 (Enolase 1 (ENO1))
- 别名
- Eno1 (ENO1 产品)
- 背景
- Eno1 also known as Alpha-Enolase, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of Eno1 is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant mouse Eno1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- 分子量
- 49.5 kDa (457aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_075608
- UniProt
- P17182
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