PKM Protein (AA 1-531) (His tag)
PKM
宿主: 小鼠
宿主: 大肠杆菌（E. Coli）
Recombinant
> 85% by SDS-PAGE
SDS, EAA
Active
1 image
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- 抗原 See all PKM 蛋白
- PKM (Pyruvate Kinase M1/2 (PKM))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-531
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PKM protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP
- 纯度
- > 85% by SDS-PAGE
- Unit Definition
- One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C
- Biological Activity Comment
- Specific activity: > 50,000pmol/min/ug. One unit will convert 1.0pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C
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Pyruvate Kinase M1/2 (PKM) protein
PKM 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, ELISA, WB, IP
Pyruvate Kinase M1/2 (PKM) proteinPKM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, ELISA, WB, IP
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl( pH 8.5) containing 0.2M NaCl, 1 mM DTT, 30 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PKM (Pyruvate Kinase M1/2 (PKM))
- 别名
- PKM (PKM 产品)
- 背景
- Pkm, also known as pyruvate kinase PKM isoform M2, is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. Pkm interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant mouse Pkm, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- 分子量
- 60.2 kDa (554aa) Confirmed by MALDI-TOF
- NCBI登录号
- NP_035229
- UniProt
- P52480
- 途径
- Warburg Effect
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