BTD Protein (AA 44-545) (His tag)
This Recombinant BTD protein is expressed in Baculovirus infected Insect Cells.
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Quick Overview for BTD Protein (AA 44-545) (His tag) (ABIN6387911)
抗原
See all BTD 蛋白
BTD
(Biotinidase (BTD))
蛋白类型
Recombinant
宿主
人
资源
Baculovirus infected Insect Cells
应用范围
SDS-PAGE (SDS)
纯度
> 85% by SDS-PAGE
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产品特性
- AA 44-545
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标记
- This BTD protein is labelled with His tag.
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序列
- AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLD IYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCM AIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAF DVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTA WMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNP VGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYD NFTLVPVWGK EGYLHVCSNG LCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLT SGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biotinidase (BTD) (AA 1-543) protein (GST tag)
BTD 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Biotinidase (BTD) protein (Myc-DYKDDDDK Tag)BTD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- BTD (Biotinidase (BTD))
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别名
- Biotinidase/BTD
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背景
- BTD, also known as biotinidase isoform 1, is a member of the nitrilase superfamily, which consists of 12 families of nitrilases, amidases, carbamylases, and N-acyltrasferases. It catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. It may have an important regulatory role in chromatin/DNA function. Recombinant human BTD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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分子量
- 57.8 kDa (510aa)
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NCBI登录号
- NP_001268652
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UniProt
- P43251
抗原
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