Our Local Distributor
北京 101111
Quick Overview for NME1 Protein (AA 1-152) (ABIN6387708)
抗原
See all NME1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 1-152
-
序列
- MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE
-
纯化方法
- > 90% by SDS-PAGE
-
内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
-
Biological Activity Comment
- Specific activity is > 1,200unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
-
-
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 1-152) protein (GST tag)
NME1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) protein (His tag)NME1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 98 % as determined by reducing SDS-PAGE.
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 1-152) proteinNME1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 2-152) protein (His tag)NME1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % as determined by SDS-PAGE.
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 2-152), (full length) protein (GST tag)NME1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % SDS, WB, ELISA
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (Transcript Variant 2) protein (His tag)NME1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NME1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NME1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Bioactivity Validated
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- 20 mM Tris-HCl buffer ( pH 7.5) containing 1 mM DTT, 10 % glycerol
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
-
-
- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
-
别名
- NM23-H1/NME1
-
背景
- Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas. Recombinant human NME1 was expressed in E. coli and purified by using conventional chromatography techniques.
-
分子量
- 17.1 kDa (152aa) confirmed by MALDI-TOF
-
NCBI登录号
- NP_000260
-
途径
- Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
抗原
-
