Galectin 9 Protein (AA 1-355) (His tag,GST tag)
Quick Overview for Galectin 9 Protein (AA 1-355) (His tag,GST tag) (ABIN6239815)
抗原
See all Galectin 9 (LGALS9) 蛋白生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-355
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标记
- This Galectin 9 protein is labelled with His tag,GST tag.
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产品特性
- Tag location: N-terminal His and GST Tag
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Biological Activity Comment
- GAL9 (Galectin-9) belongs to the galectin family, which is defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc). It is reported that GAL9 induces T-helper type 1 lymphocyte (Th1) death by binding to HAVCR2 (Hepatitis A virus cellular receptor 2); besides, the interaction between GAL9 and PDI (Protein disulfide-isomerase) leads to disulfide reductase activity increasing at the plasma membrane, therefore alters the plasma membrane redox state and enhances cell migration. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GAL9 with recombinant human HAVCR2 and recombinant human PDI separately. Briefly, GAL9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HAVCR2-coated and PDI-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GAL9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of GAL9 with HAVCR2 and PDI were shown in Figure 1 and Figure 2, and this effect was in a dose dependent manner The binding activity of GAL9 with HAVCR2. Figure 2. The binding activity of GAL9 with PDI.
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Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-355) protein (His tag)
custom-made LGALS9 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-353) protein (His tag)custom-made LGALS9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 254-355) protein (GST tag)LGALS9 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) protein (GST tag)LGALS9 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % as determined by reducing SDS-PAGE.
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) protein (GST tag)LGALS9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 2-323) protein (His tag)LGALS9 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-148) protein (His tag)LGALS9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-183) protein (His-IF2DI Tag)LGALS9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) proteinLGALS9 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
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应用备注
- Isoelectric Point: 9.3
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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储存液
- Dithiothreitol (DTT), Other preservative, ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))
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别名
- Galectin 9 (GAL9)
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背景
- Alternative Names: LGALS9, Ecalectin, Lectin,Galactoside-Binding Soluble 9, Tumor antigen HOM-HD-21
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分子量
- 69kDa
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UniProt
- O00182
抗原
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