JAK2 Protein (AA 752-1132, partial) (His tag)
Quick Overview for JAK2 Protein (AA 752-1132, partial) (His tag) (ABIN5709779)
抗原
See all JAK2 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 752-1132, partial
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标记
- This JAK2 protein is labelled with His tag.
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序列
- KPLSALDSQR KLQFYEDRHQ LPAPKWAELA NLINNCMDYE PDFRPSFRAI IRDLNSLFTP DYELLTENDM LPNMRIGALG FSGAFEDRDP TQFEERHLKF LQQLGKGNFG SVEMCRYDPL QDNTGEVVAV KKLQHSTEEH LRDFEREIEI LKSLQHDNIV KYKGVCYSAG RRNLKLIMEY LPYGSLRDYL QKHKERIDHI KLLQYTSQIC KGMEYLGTKR YIHRDLATRN ILVENENRVK IGDFGLTKVL PQDKEYYKVK EPGESPIFWY APESLTESKF SVASDVWSFG VVLYELFTYI EKSKSPPAEF MRMIGNDKQG QMIVFHLIEL LKNNGRLPRP DGCPDEIYMI MTECWNNNVN QRPSFRDLAL RVDQIRDNMA G
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纯化方法
- SDS-PAGE
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 106,314.67 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 172,528.32 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Janus Kinase 2 (JAK2) (AA 1-1132) protein (His tag)
custom-made JAK2 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Janus Kinase 2 (JAK2) (AA 1014-1132) protein (His tag)JAK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Janus Kinase 2 (JAK2) (AA 241-351) protein (His-IF2DI Tag)JAK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1-2 mg/mL
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缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
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储存条件
- -80 °C,4 °C,-20 °C
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储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- JAK2 (Janus Kinase 2 (JAK2))
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别名
- JAK2
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背景
- Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO), or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
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分子量
- 48.7 kDa
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UniProt
- O60674
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途径
- JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response
抗原
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