我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

EGFR Protein (AA 25-645) (His tag,AVI tag,Biotin)

Name: EGFR Protein (AA 25-645) (His tag,AVI tag,Biotin)
SKU: ABIN5674594
Availability: OutOfStock

This Recombinant EGFR protein is expressed in HEK-293 Cells and has been mentioned in 3+ publications.

Biotinylated Human EGF R, His,Avitag on under reducing (R) condition. (EGFR Protein (AA 25-645) (His tag,AVI tag,Biotin))

SDS

Immobilized Human EGF, Tag Free at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His,Avitag (ABIN5674594,ABIN6253697) with a linear range of 0. (EGFR Protein (AA 25-645) (His tag,AVI tag,Biotin))

ELISA

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His,Avitag (ABIN5674594,ABIN6253697) with a linear range of 2-6 ng/mL (QC tested). (EGFR Protein (AA 25-645) (His tag,AVI tag,Biotin))

ELISA

3 images

(3 references)

产品编号 ABIN5674594

发货至: 中国

Quick Overview for EGFR Protein (AA 25-645) (His tag,AVI tag,Biotin) (ABIN5674594)

抗原

See all EGFR 蛋白

EGFR (Epidermal Growth Factor Receptor (EGFR))

蛋白类型

Recombinant

生物活性

Active

宿主

人

资源

HEK-293 Cells

纯度

>90 % as determined by SDS-PAGE.

Discover our top product EGFR 蛋白

产品特性

AA 25-645

标记

This EGFR protein is labelled with His tag,AVI tag,Biotin.

品牌

PrecisionAvi

序列

AA 25-645

特异性

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

产品特性

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 72.3 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素水平

Less than 1.0 EU per μg by the LAL method.
想要此Protein的其他选项吗？

!

探索我们的预定义定制蛋白和定制蛋白服务！

您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案

Epidermal Growth Factor Receptor (EGFR) (AA 25-645) protein (His-Avi Tag)
EGFR 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor Receptor (EGFR) (AA 25-645) protein (His-Avi Tag,Biotin)
EGFR 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor Receptor (EGFR) (AA 25-645) protein (Fc Tag)
EGFR 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Epidermal Growth Factor Receptor (EGFR) (AA 25-645) (Active) protein (Fc Tag)
EGFR 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Epidermal Growth Factor Receptor (EGFR) (AA 25-378) (Active) protein (His tag,AVI tag,Biotin)
EGFR 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active

Epidermal Growth Factor Receptor (EGFR) (AA 25-645) protein (His tag)
EGFR 宿主: 猕猴宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor Receptor (EGFR) (AA 25-647) protein (His tag)
EGFR 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor Receptor (EGFR) (AA 1-405) protein (GST tag)
EGFR 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

Epidermal Growth Factor Receptor (EGFR) (AA 25-645) protein (His tag)
EGFR 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA

Epidermal Growth Factor Receptor (EGFR) (AA 25-645) protein (His tag)
EGFR 宿主: Cynomolgus 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

说明

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4

注意事项

Please avoid repeated freeze-thaw cycles.

储存条件

-20 °C
Tang, Zheng, Melo, Mabardi, Castaño, Xie, Li, Kudchodkar, Wong, Jeng, Maus, Irvine: "Enhancing T cell therapy through TCR-signaling-responsive nanoparticle drug delivery." in: Nature biotechnology, Vol. 36, Issue 8, pp. 707-716, (2019) (PubMed).

Choi, Yu, Castano, Bouffard, Schmidts, Larson, Bailey, Boroughs, Frigault, Leick, Scarfò, Cetrulo, Demehri, Nahed, Cahill, Wakimoto, Curry, Carter, Maus: "CAR-T cells secreting BiTEs circumvent antigen escape without detectable toxicity." in: Nature biotechnology, (2019) (PubMed).

Yu, Pegram, Bigner, Chandramohan: "Development and validation of a cell-based fluorescent method for measuring antibody affinity." in: Journal of immunological methods, Vol. 442, pp. 49-53, (2017) (PubMed).
抗原

EGFR (Epidermal Growth Factor Receptor (EGFR))

别名

EGF R

背景

The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

分子量

72.3 kDa

NCBI登录号

NP_005219

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins