CD14 Protein (CD14) (His tag)
Quick Overview for CD14 Protein (CD14) (His tag) (ABIN5663349)
抗原
See all CD14 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This CD14 protein is labelled with His tag.
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序列
- MERASCLLLL LLPLVHVSAT TPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV PACARSTLSV G VSGTLVLLQG ARGFA
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产品特性
- The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.
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纯化方法
- affinity chromatography with monoclonal CD14 antibody biG2
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内毒素水平
- <1EU/μg of recombinant human CD14 as determined by LAL method
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Biological Activity Comment
- Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5μg/ml) to 6 x 105 CD14+CHO transfectants (FACS)
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CD14 (CD14) (AA 16-345) protein (His tag)
CD14 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 1-375) protein (GST tag)CD14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD14 (CD14) (AA 20-352) protein (His tag)CD14 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD14 (CD14) (AA 20-171) protein (His-IF2DI Tag)CD14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CD14 (CD14) (AA 20-352) (Active) protein (His tag)CD14 宿主: 人 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
CD14 (CD14) (AA 18-345) (Active) protein (His-Fc tag)CD14 宿主: 小鼠 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
CD14 (CD14) (AA 20-344) protein (His tag)CD14 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD14 (CD14) (AA 20-349) protein (His tag)CD14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
CD14 (CD14) protein (His tag,Fc Tag)CD14 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL
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浓度
- 0.25 mg/mL
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缓冲液
- sterile-filtered phosphate balanced salt solution, pH 7.2
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储存液
- Without preservative
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储存条件
- RT,-20 °C,-80 °C
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储存方法
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Storage: -20°C or -70°C
Storage/Stability: Long time storage at -70oC. When stored at recommended temperature, this protein is stable for 12 months. The lyophilized product thought stable room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long-term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided !!!
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有效期
- 12 months
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- CD14
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别名
- CD14
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背景
- Background: CD14 acts as a receptor for endotoxin (LPS), expressed strongly on monocytes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.The rhuCD14 was produced in human CD14 transfected CHO-cells in serum free medium and has been purified by affinity chromatography with monoclonal antibody to hCD14 biG2at column and controlled by SDS page (90-95 % purity). Before transfection, the complete human CD14-cDNA amplified by PCR and cloned into expression vector p-POL-DHFR. CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, also expressed by most tissue macrophages.
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分子量
- ~50kDa
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基因ID
- 929
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NCBI登录号
- NP_000582
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UniProt
- P08571
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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