ERK1 Protein (double Mutant)
Quick Overview for ERK1 Protein (double Mutant) (ABIN5624588)
抗原
See all ERK1 (MAPK3) 蛋白蛋白类型
宿主
资源
应用范围
-
-
产品特性
- double Mutant
-
Supplier Product No.
- 009-001-gr1
-
Supplier
- Rockland
-
原理
- ERK1 double mutant human recombinant protein
-
产品特性
- MAPK3, ERK, ERK1, PRKM3, ERK-1 recombinant protein
-
纯化方法
- ERK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 90% pure.
-
过滤
- Sterile filtered
-
-
Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) protein (GST tag)
MAPK3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-379) protein (GST tag)MAPK3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-379) protein (His tag)MAPK3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
-
-
应用备注
-
Application Note: Human ERK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical ERK1 phosphorylated T202 or Y204 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
Other: Dot Blot
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
-
别名
- MAPK3
-
背景
-
Synonyms: MAPK3, ERK, ERK1, PRKM3, ERK-1 recombinant protein
Background: ERK1 isoform double mutant recombinant protein is modified to put alanine residues at two key activation sites, Threonine 202 and Tyrosine 204. Mitogen activated protein kinase 3, also known as MAPK3, ERK, or ERK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. The inactive double mutant ERK1 recombinant protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
-
UniProt
- P27361
-
途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
抗原
-
