TNFRSF4 Protein (AA 29-216) (His tag,AVI tag,Biotin)
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Quick Overview for TNFRSF4 Protein (AA 29-216) (His tag,AVI tag,Biotin) (ABIN5526608)
抗原
See all TNFRSF4 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 29-216
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标记
- This TNFRSF4 protein is labelled with His tag,AVI tag,Biotin.
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品牌
- MABSol® - PrecisionAvi
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序列
- AA 29-216
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特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
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产品特性
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This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 22.8 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
This product is biotinylated with precision by using the Avitag™ technology, resulting in precise, single residue biotinylation at the lysine of the tag. This leads to minimal interence with protein binding, structure, and function, as well as uniform orientation upon immobilization. -
内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. -
限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- PBS, pH 7.4
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注意事项
- Please avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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- TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
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别名
- OX40
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背景
- Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
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分子量
- 22.8 kDa
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NCBI登录号
- NP_003318
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途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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