TRIB3 Protein (AA 1-358) (Strep Tag)
Quick Overview for TRIB3 Protein (AA 1-358) (Strep Tag) (ABIN3096074)
抗原
See all TRIB3 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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产品特性
- AA 1-358
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标记
- This TRIB3 protein is labelled with Strep Tag.
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序列
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MRATPLAAPA GSLSRKKRLE LDDNLDTERP VQKRARSGPQ PRLPPCLLPL SPPTAPDRAT AVATASRLGP YVLLEPEEGG RAYQALHCPT GTEYTCKVYP VQEALAVLEP YARLPPHKHV ARPTEVLAGT QLLYAFFTRT HGDMHSLVRS RHRIPEPEAA VLFRQMATAL AHCHQHGLVL RDLKLCRFVF ADRERKKLVL ENLEDSCVLT GPDDSLWDKH ACPAYVGPEI LSSRASYSGK AADVWSLGVA LFTMLAGHYP FQDSEPVLLF GKIRRGAYAL PAGLSAPARC LVRCLLRREP AERLTATGIL LHPWLRQDPM PLAPTRSHLW EAAQVVPDGL GLDEAREEEG DREVVLYG
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.. -
产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
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纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Baculovirus infected Insect CellsConjugate GST tagOrigin HumanPrice 5,818.62 ¥表达系统 Wheat germConjugate GST tagOrigin HumanPrice 6,623.29 ¥表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 20,380.77 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Tribbles Homolog 3 (Drosophila) (TRIB3) (AA 1-354) protein (His tag)
custom-made TRIB3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tribbles Homolog 3 (Drosophila) (TRIB3) (AA 1-358) protein (His tag)custom-made TRIB3 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tribbles Homolog 3 (Drosophila) (TRIB3) protein (GST tag)TRIB3 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Tribbles Homolog 3 (Drosophila) (TRIB3) (AA 1-358) protein (GST tag)TRIB3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. -
注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- TRIB3 (Tribbles Homolog 3 (Drosophila) (TRIB3))
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别名
- TRIB3
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背景
- Tribbles homolog 3 (TRB-3) (Neuronal cell death-inducible putative kinase) (SINK) (p65-interacting inhibitor of NF-kappa-B),FUNCTION: Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed:15781252, PubMed:15775988). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed:15781252, PubMed:15775988). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed:15781252, PubMed:15775988). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed:12736262). Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed:15299019). Can inhibit APOBEC3A editing of nuclear DNA (PubMed:22977230). {ECO:0000250|UniProtKB:Q8K4K2, ECO:0000269|PubMed:12736262, ECO:0000269|PubMed:15299019, ECO:0000269|PubMed:15775988, ECO:0000269|PubMed:15781252, ECO:0000269|PubMed:22977230}.
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分子量
- 39.6 kDa
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UniProt
- Q96RU7
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
抗原
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