WISP3 Protein (Transcript Variant 1)
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- 抗原 See all WISP3 蛋白
- WISP3 (Wnt1 Inducible Signaling Pathway Protein 3 (WISP3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human WISP3 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 ug/ml.
- Top Product
- Discover our top product WISP3 蛋白
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Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (Active) protein
WISP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (AA 1-372) protein (GST tag)WISP3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (AA 24-354) protein (His tag)Crystallography grade WISP3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- WISP3 (Wnt1 Inducible Signaling Pathway Protein 3 (WISP3))
- 别名
- Wisp3 (WISP3 产品)
- 别名
- CCN6 Protein, LIBC Protein, PPAC Protein, PPD Protein, ENSMUSG00000062074 Protein, Gm735 Protein, WISP-3 Protein, RGD1564120 Protein, WISP3 Protein, si:dkey-119m7.6 Protein, WNT1 inducible signaling pathway protein 3 Protein, WISP3 Protein, Wisp3 Protein, wisp3 Protein
- 背景
- This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene.
- 分子量
- 36.8 kDa
- NCBI登录号
- NP_003871
- 途径
- WNT signaling, Growth Factor Binding
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