WISP3 Protein (AA 24-354)
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北京 101111
Quick Overview for WISP3 Protein (AA 24-354) (ABIN7866104)
抗原
See all WISP3 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 24-354
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原理
- Human WISP3 Protein Recombinant
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序列
- TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCRE PGDIFSELKI L
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产品特性
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Protein Size: Recombinant WISP3 migrates as an approximately 36 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human WISP3 mature chain including a C-terminal 6xHis tag was expressed in E. Coli. Recombinant Human WNT1-inducible-signaling pathway protein 3 encodes amino acid residues 24-354.
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内毒素水平
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 μg/ml.
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Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (Active) protein
WISP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (AA 1-372) protein (GST tag)WISP3 宿主: 人 宿主: 小麦胚 Recombinant AA, AP, ELISA, WB
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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缓冲液
- WISP3 was Lyophilized from PBS, pH 7.2.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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有效期
- 24 months
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- WISP3 (Wnt1 Inducible Signaling Pathway Protein 3 (WISP3))
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别名
- WISP3
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分子量
- 36 kDa
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途径
- WNT signaling, Growth Factor Binding
抗原
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